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<t>Compound-level</t> activity across feature spaces. The heatmap shows compound-level activity significance for CellPaintSSL, OpenPhenom, and uniDINO. In this scenario, compound-treated well profiles served as queries, replicate wells of the same compound were considered positives, and dimethyl sulfoxide <t>(DMSO)</t> wells were considered negatives. Color represents −log10 of the adjusted p -value, while gray cells indicate non-significant compound–feature-space pairs. Compounds are ordered by their activity classification pattern across feature spaces: shared3 denotes compounds significant in all three feature spaces, shared2 denotes compounds significant in exactly two feature spaces, unique denotes compounds significant in only one feature space, and inactive denotes compounds not classified as activity-positive in any feature space. Within each block, compounds are ordered by the summed −log10 adjusted p -value across the three feature spaces, from higher to lower values.
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<t>Compound-level</t> activity across feature spaces. The heatmap shows compound-level activity significance for CellPaintSSL, OpenPhenom, and uniDINO. In this scenario, compound-treated well profiles served as queries, replicate wells of the same compound were considered positives, and dimethyl sulfoxide <t>(DMSO)</t> wells were considered negatives. Color represents −log10 of the adjusted p -value, while gray cells indicate non-significant compound–feature-space pairs. Compounds are ordered by their activity classification pattern across feature spaces: shared3 denotes compounds significant in all three feature spaces, shared2 denotes compounds significant in exactly two feature spaces, unique denotes compounds significant in only one feature space, and inactive denotes compounds not classified as activity-positive in any feature space. Within each block, compounds are ordered by the summed −log10 adjusted p -value across the three feature spaces, from higher to lower values.
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Compound-level activity across feature spaces. The heatmap shows compound-level activity significance for CellPaintSSL, OpenPhenom, and uniDINO. In this scenario, compound-treated well profiles served as queries, replicate wells of the same compound were considered positives, and dimethyl sulfoxide (DMSO) wells were considered negatives. Color represents −log10 of the adjusted p -value, while gray cells indicate non-significant compound–feature-space pairs. Compounds are ordered by their activity classification pattern across feature spaces: shared3 denotes compounds significant in all three feature spaces, shared2 denotes compounds significant in exactly two feature spaces, unique denotes compounds significant in only one feature space, and inactive denotes compounds not classified as activity-positive in any feature space. Within each block, compounds are ordered by the summed −log10 adjusted p -value across the three feature spaces, from higher to lower values.

Journal: International Journal of Molecular Sciences

Article Title: Retrieval-Based Evaluation of Cell Painting Feature Spaces Reveals Differences in the Preservation of Biologically Meaningful Phenotypic Similarity

doi: 10.3390/ijms27135727

Figure Lengend Snippet: Compound-level activity across feature spaces. The heatmap shows compound-level activity significance for CellPaintSSL, OpenPhenom, and uniDINO. In this scenario, compound-treated well profiles served as queries, replicate wells of the same compound were considered positives, and dimethyl sulfoxide (DMSO) wells were considered negatives. Color represents −log10 of the adjusted p -value, while gray cells indicate non-significant compound–feature-space pairs. Compounds are ordered by their activity classification pattern across feature spaces: shared3 denotes compounds significant in all three feature spaces, shared2 denotes compounds significant in exactly two feature spaces, unique denotes compounds significant in only one feature space, and inactive denotes compounds not classified as activity-positive in any feature space. Within each block, compounds are ordered by the summed −log10 adjusted p -value across the three feature spaces, from higher to lower values.

Article Snippet: After 24 h, 10 μL of either DMSO vehicle (MedChemExpress, Shanghai, China) or compound solution was added to each well.

Techniques: Activity Assay, Blocking Assay